Download centos7 image and make U disk boot disk
Download from Tsinghua mirroring
wget https://mirrors.tuna.tsinghua.edu.cn/centos/7.7.1908/isos/x86_64/CentOS-7-x86_64-DVD-1908.iso
Set U disk as the first boot entry
F9 boot can enter the BIOS setup
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System environment to build
System version as follows:
CentOS Linux Release 7.7.1908 (Core)
Centos interface can not enter the system after the installation is complete, using the following command to the command line
ctrl alt F2 into the command line, enter a user name and password
- Open network, and set the automatic connection
cd /etc/sysconfig/network-scripts
ifup ifcfg-enp4s0f2
- Installation R 3.6.1
yum install R
R installation of the package
need_packages=c("reshape2","RColorBrewer","ggplot2","plyr","grid","Mfuzz","WGCNA","pheatmap")
install.packages(need_packages)
BGI need R package
if (!requireNamespace("BiocManager", quietly = TRUE))
install.packages("BiocManager")
BiocManager::install(version = "3.10")
BiocManager::install("AnnotationDbi","EBSeq","NOISeq","DEGseq","clusterProfiler")
BiocManager::install("topGO")
BiocManager::install("Rgraphviz")
BiocManager::install(c("impute","preprocessCore")) #WGCNA
- DESeq2 installation error
jpeglib.h: No such file or directory
Installation system relies on the following
yum install -y libjpeg-devel
- Install perl modules
GD manually install modules, being given after installation
wget http://www.cpan.org/authors/id/L/LD/LDS/GD-1.16.tar.gz
cd GD-1.16
perl Makefile.PL
make
make install
- Install gd dependent, you may still find some problems, consider installing cpan
yum install -y perl-GD
yum install -y gd-devel
- Finally, use cpan installation
cpan install YAML
cpan install Module:Build
cpan install Excel::Writer::XLSX
- Installation module
参考module安装日志
- Installation and set up the source image conda
channels:
- https://mirrors.tuna.tsinghua.edu.cn/anaconda/cloud/conda-forge/
- https://mirrors.tuna.tsinghua.edu.cn/anaconda/pkgs/free/
- defaults
- r
show_channel_urls: true
conda config --add channels https://mirrors.tuna.tsinghua.edu.cn/anaconda/pkgs/free/
conda config --add channels https://mirrors.tuna.tsinghua.edu.cn/anaconda/pkgs/main/
conda config --add channels https://mirrors.tuna.tsinghua.edu.cn/anaconda/cloud/conda-forge/
conda config --add channels https://mirrors.tuna.tsinghua.edu.cn/anaconda/cloud/bioconda/
/home/yueyao/bio/tools/miniconda/4.5.1/bin/conda create -p /home/yueyao/bio/tools/snakemake/3.13.3 snakemake
- Installation texlive
参考texlive安装日志
- Installation fastqc
wget fastqc_v0.11.8.zip
unzip fastqc_v0.11.8.zip
mkdir -p /usr/local/Modules/modulefiles/fastqc/0.11.8