基于PASA进行基因预测

PASA, acronym for Program to Assemble Spliced Alignments, is a eukaryotic genome annotation tool that exploits spliced alignments of expressed transcript sequences to automatically model gene structures, and to maintain gene structure annotation consistent with the most recently available experimental sequence data. PASA also identifies and classifies all splicing variations supported by the transcript alignments.

Note:
Combine genome and Trinity de novo RNA-Seq assemblies to generate a comprehensive transcript database.

基于RNA-seq数据，将其利用trinity组装---》利用PASA将组装好的序列比对到draft ref----〉预测基因

软件安装

• Trinity

1 conda create -n trinity trinity=2.8.5
2 # 激活环境
3 conda activate trinity